@article{Xu2025, 
author = {Na Xu and Dong Yu and Longhui Zhang and Tiankang Wang and Zhongxiao Hu and Quanqiang Lei and Yingxin Qiu and Zheming Yuan and Shufeng Song and Li Li},
title = {Establishment of a novel engineering breeding technology for cytoplasmic male sterility},
year = {2025},
journal = {The Crop Journal},
volume = {13},
number = {4},
pages = {1305-1311},
keywords = {Three-line hybrid rice, CMS-WA, Rf gene, CEBT, Reproductive lines},
url = {https://www.sciopen.com/article/10.1016/j.cj.2025.04.010},
doi = {10.1016/j.cj.2025.04.010},
abstract = {The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20, sorting marker gene DsRed2, and pollen lethal gene ZmAA1, then transformed this into a CMS line. We obtained reproductive lines without alterations to main agronomic traits, which can produce engineering sterile lines (ESL) and reproductive line seeds at a 1:1 ratio by self-crossing. CEBT converts the traditional three-line system into an efficient ‘new two-line’ hybrid framework: the reproductive line is improved while simultaneously improving the ESL, which significantly shortens the breeding process for three-line hybrid rice.}
}