TY  - JOUR
AU  - Xu, Na
AU  - Yu, Dong
AU  - Zhang, Longhui
AU  - Wang, Tiankang
AU  - Hu, Zhongxiao
AU  - Lei, Quanqiang
AU  - Qiu, Yingxin
AU  - Yuan, Zheming
AU  - Song, Shufeng
AU  - Li, Li
PY  - 2025
TI  - Establishment of a novel engineering breeding technology for cytoplasmic male sterility
JO  - The Crop Journal
SN  - 2095-5421
SP  - 1305
EP  - 1311
VL  - 13
IS  - 4
AB  - The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20, sorting marker gene DsRed2, and pollen lethal gene ZmAA1, then transformed this into a CMS line. We obtained reproductive lines without alterations to main agronomic traits, which can produce engineering sterile lines (ESL) and reproductive line seeds at a 1:1 ratio by self-crossing. CEBT converts the traditional three-line system into an efficient ‘new two-line’ hybrid framework: the reproductive line is improved while simultaneously improving the ESL, which significantly shortens the breeding process for three-line hybrid rice.
UR  - https://doi.org/10.1016/j.cj.2025.04.010
DO  - 10.1016/j.cj.2025.04.010