@article{Xu2025, 
author = {Lei Xu and Shijun Fan and Kailai Huang and Hang Qian and Huan Ye and Yi Xi and Yingli Liang and Kai He and Meng Li and Bin Tu and Weilan Chen and Bingtian Ma and Hua Yuan and Shigui Li and Peng Qin and Yuping Wang},
title = {qLTS5/OsALA4 encodes a P4-ATPase that positively regulates cold tolerance in rice seedlings},
year = {2025},
journal = {The Crop Journal},
volume = {13},
number = {4},
pages = {1032-1040},
keywords = {Rice, Abiotic stress, Cold stress, Plasma membrane fluidity},
url = {https://www.sciopen.com/article/10.1016/j.cj.2025.05.014},
doi = {10.1016/j.cj.2025.05.014},
abstract = {Rice (Oryza sativa L.), a thermophilic crop, is highly sensitive to cold stress, particularly during the seedling stage. Developing cold-tolerant rice varieties is a possible strategy to mitigate yield losses caused by low temperatures. However, few genes for cold tolerance have been identified. In this study, we identified OsALA4 (Aminophospholipid ATPase 4), encoding a plasma membrane-localized P4-ATPase, from a chromosomal segment substitution line (CSSL-K2832-2) harboring cold-tolerance QTL qLTS5 (Low Temperature Sensitive 5). Genetic and subcellular localization analyses revealed that OsALA4 regulates cold tolerance by maintaining plasma membrane fluidity and cellular homeostasis. Physiological assessments showed that OsALA4 reduces malondialdehyde (MDA), electrolyte leakage, reactive oxygen species (ROS), and cell death under cold stress. Promoter activity assays indicated that stronger OsALA4 expression in Nipponbare (OsALA4Nip) correlated with enhanced cold tolerance. Further experiments demonstrated that SNP sites within the promoter regions (−1500 bp to −700 bp) of OsALA4Nip and OsALA49311 influenced their activity. This study highlights OsALA4 as a valuable genetic target for breeding cold tolerant rice.}
}