@article{LI2023, 
author = {Jiahui LI and Wenya JIAO and Qiuxia JIN and Si MI and Yunhe LIAN and Yaxin SANG and Xianghong WANG},
title = {Analysis of Phenylpropanoid Components and Antioxidant and Uric Acid-Lowering Activities of Ginger},
year = {2023},
journal = {Food Science},
volume = {44},
number = {11},
pages = {26-31},
keywords = {dried ginger, ferulic acid, p-coumaric acid, antioxidant activity, xanthine oxidase, molecular docking},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20220621-221},
doi = {10.7506/spkx1002-6630-20220621-221},
abstract = {In order to explore the biological functions of active ingredients in dried ginger, high performance liquid chromatography (HPLC) and mass spectrometry (MS) with selective reaction monitoring/multi-reaction monitoring (SRM/MRM) were used for qualitative and quantitative analysis of active ingredients in dried ginger. The antioxidant and uric acid-lowering activities of dried ginger extract and its active components were determined. The results showed that the most abundant phenylpropanoid components in dried ginger extract were phenylalanine, ferulic acid and p-coumaric acid. All these compounds had significant antioxidant capacity in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging capacity and ferric ion reducing power, and the decreasing order of the antioxidant capacity was ferulic acid > dried ginger extract > p-coumaric acid. In vitro xanthine oxidase inhibition assay showed that all the three drugs had strong uric acid lowering activity, the effect of p-coumaric acid being more pronounced than that of ferulic acid and dried ginger extract. Molecular docking revealed that ferulic acid and p-coumaric acid inhibited xanthine oxidase by the formation of hydrogen bonds, van der Waals force and π-π stacking with amino acid residues in the enzyme. In this study, ferulic acid, p-coumaric acid and ginger extract have good antioxidant capacity and xanthine oxidase inhibitory activity in vitro, and therefore have the potential to be developed into new uric acid-lowering products.}
}