@article{XU2023, 
author = {Yang XU and Jiaying XIN and Huimin LI and Guiru WANG and Yuqing WANG},
title = {Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol},
year = {2023},
journal = {Food Science},
volume = {44},
number = {14},
pages = {100-105},
keywords = {lipase activity, gold nanocluster, fluorescence inner filter effect, optical properties, detection},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20220815-169},
doi = {10.7506/spkx1002-6630-20220815-169},
abstract = {Lipase activity was detected based on the fluorescence properties of gold nanoclusters (AuNCs) and the inner filter effect (IFE) of p-nitrophenol. Glutathione-modified AuNCs was used as fluorophore, and p-nitrophenol produced from the lipase-catalyzed hydrolysis of p-nitrophenol palmitate as fluorescence absorbent. The results showed that under the optimal conditions (p-nitrophenyl palmitate concentration 1.6 mg/mL, pH 7.5, temperature 50 ℃, and reaction time 20 min), the relative fluorescence intensity (F0–F, y) was positively correlated with lipase activity (x) in the range of 5.6‒196 U/L, which was described by the equation y =2.0035+0.9368x, with a correlation coefficient (r2) of 0.9978, and the detection limit was 1.3 U/L (at a signal-to-noise ratio of 3). The developed method is simple and sensitive, and can be applied to the detection of lipase activity.}
}