@article{KOU2023, 
author = {Xiuying KOU and Baoqing ZHOU and Ling CHEN and Jingsha DAI and Jumei ZHANG and Qingping WU},
title = {Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)},
year = {2023},
journal = {Food Science},
volume = {44},
number = {7},
pages = {313-323},
keywords = {clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein, foodborne pathogens, trans-cleavage, rapid detection technology},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20220316-195},
doi = {10.7506/spkx1002-6630-20220316-195},
abstract = {The early screening and rapid detection of foodborne pathogens is critical to food safety and public health. However, the traditional detection method is cumbersome, time-consuming and laborious, and cannot meet the requirement of rapid detection. CRISPR/Cas, composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas), is an immune system widely present in bacteria and archaea, which provides a novel pathway for low-cost, rapid, specific and sensitive detection of foodborne pathogens due to its efficient and specific sequence recognition and cleavage activity. This review article introduces the principle, mechanism and development of the CRISPR/Cas system, and summarizes the latest progresses in the rapid detection of foodborne pathogens based on the CRISPR/Cas system combined with different result reporting methods in recent years. Its advantages, limitations and future development prospects are also discussed.}
}