@article{CAI2023, 
author = {Xixi CAI and Chen LI and Xu CHEN and Yuannan HUANG and Caili FU and Shaoyun WANG},
title = {Protective Effect of Functional Peptides Derived from Crimson Snapper Scales on Oxidative Stress Damage in Caco-2 Cells},
year = {2023},
journal = {Food Science},
volume = {44},
number = {7},
pages = {10-17},
keywords = {antioxidant, cell apoptosis, Caco-2 cells, oxidative stress damage, marine-derived peptides},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20221112-144},
doi = {10.7506/spkx1002-6630-20221112-144},
abstract = {The purpose of this study was to investigate the effect of crimson snapper scale peptides (CSSPs) on H2O2-induced oxidative damage in Caco-2 cells. The levels of lactate dehydrogenase (LDH) and interleukin-8 (IL-8) in the cell supernatant, the activity of intracellular antioxidant enzymes, the release of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA), and the expression of apoptosis-related genes (Caspase-3, Caspase-9, Bax and Bcl-2) were measured. The antioxidant effects of CSSPs in intestinal cells were elucidated from the aspects of cellular redox state and apoptosis. Results showed that CSSPs mitigated H2O2-induced oxidative damage in Caco-2 cells through the antioxidant defense system. This effect involved increased activities of antioxidant enzymes and consequent inhibition of LDH release, intracellular ROS accumulation, and MDA and IL-8 production. Furthermore, CSSPs inhibited oxidative stress-induced apoptosis by regulating the mitochondrial apoptotic pathway, which significantly increased mitochondrial membrane potential, down-regulated the mRNA expression of Caspase-3, Caspase-9 and Bax, and up-regulated the mRNA expression of Bcl-2. In conclusion, CSSPs can effectively reduce the degree of cell damage by maintaining redox homeostasis and inhibiting apoptosis.}
}