@article{DU2023, 
author = {Juan DU and Xin CHEN and Kai LIU and Jialei LIU and Pengjie LUO and Dong LIANG and Yanhong BAI},
title = {Highly Sensitive Colorimetric Detection of Listeria monocytogenes by Using Gold Nanoparticles Based on Magnetic Metal-Organic Framework Separation},
year = {2023},
journal = {Food Science},
volume = {44},
number = {6},
pages = {360-367},
keywords = {gold nanoparticles, magnetic separation, Listeria monocytogenes, colorimetric assay},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20220316-179},
doi = {10.7506/spkx1002-6630-20220316-179},
abstract = {In this study, magnetic metal organic framework composites as capture probe and immunofunctionalized gold nanoparticles as signal probe were used to develop a rapid colorimetric method for the detection of Listeria monocytogenes. Under optimal conditions, the visual detection limit for L. monocytogenes was 1.2 × 103 CFU/mL. The results of ultraviolet spectroscopy showed a good linear relationship in the range of 1.2 × 101-1.2 × 108 CFU/mL (Y = 0.519 - 0.043X, R2 = 0.978), the detection limit was as low as 0.45 CFU/mL, and the intra-batch coefficient of variation of all concentration gradients was between 0.31% and 1.30%. The recoveries for spiked chicken breast were 91.1%-108.7% with coefficient of variation less than 7.4%, and the result was consistent with that of the plate counting method. In conclusion, the proposed assay is rapid, accurate sensitive, and highly applicable to the detection of foodborne pathogens.}
}