@article{HE2023, 
author = {Jianju HE and Xinxin LIU and Hongling SHI and Luni RAN and Xian WANG and Yingjun ZHANG and Cunduo TANG},
title = {Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae},
year = {2023},
journal = {Food Science},
volume = {44},
number = {16},
pages = {192-197},
keywords = {nicotinamide nucleoside kinase, β-nicotinamide mononucleotide, expression, identification, biocatalysis},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20220912-095},
doi = {10.7506/spkx1002-6630-20220912-095},
abstract = {In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. The enzyme activity in the fermentation broth was 14.75 IU/mL, and the specific activity the purified enzyme was 2252.59 IU/mg. In addition, the kinetic parameters of ScNRK1 were significantly higher than those of other reported nicotinamide nucleoside kinases, so ScNRK1 could be more advantageous in the enzymatic synthesis of nicotinamide mononucleotide (NMN).}
}