TY  - JOUR
AU  - HE, Jianju
AU  - LIU, Xinxin
AU  - SHI, Hongling
AU  - RAN, Luni
AU  - WANG, Xian
AU  - ZHANG, Yingjun
AU  - TANG, Cunduo
PY  - 2023
TI  - Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae
JO  - Food Science
SN  - 1002-6630
SP  - 192
EP  - 197
VL  - 44
IS  - 16
AB  - In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. The enzyme activity in the fermentation broth was 14.75 IU/mL, and the specific activity the purified enzyme was 2252.59 IU/mg. In addition, the kinetic parameters of ScNRK1 were significantly higher than those of other reported nicotinamide nucleoside kinases, so ScNRK1 could be more advantageous in the enzymatic synthesis of nicotinamide mononucleotide (NMN).
UR  - https://doi.org/10.7506/spkx1002-6630-20220912-095
DO  - 10.7506/spkx1002-6630-20220912-095