@article{MA2023, 
author = {Yan MA and Zhongjiang WANG and Jingyu YANG and Zhe LI and Xia PENG and Xiufeng SHAN and Bailiang LI and Weiwei MA},
title = {Alleviative Effect of Bifidobacterium animalis subsp. lactis XLTG11 on Antibiotic-Associated Diarrhea Induced by Clindamycin},
year = {2023},
journal = {Food Science},
volume = {44},
number = {3},
pages = {170-178},
keywords = {Bifidobacterium animalis subsp. lactis XLTG11, antibiotic-associated diarrhea, gut microbiota, short-chain fatty acids, nuclear factor κB signaling pathway},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20211118-232},
doi = {10.7506/spkx1002-6630-20211118-232},
abstract = {Objective   To investigate the relieving effect of Bifidobacterium animalis subsp. lactis XLTG11 on antibiotic-associated diarrhea (AAD) in mice using clindamycin-induced AAD model. Methods   Forty-eight 6-week-old C57BL/6N male mice were randomly divided into four groups: normal control, model, low-dose and high-dose XLTG11. All mice except for the control group were administered with clindamycin orally daily for 14 days to induce AAD, The low-dose and high-dose groups were given 0.2 mL of the bacterial suspensions with viable count of 5×106 and 1×107 CFU, respectively. Body mass gain, cecum mass, fecal water content and fecal consistency score were measured. The levels of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), IL-1β, and IL-10 in cecum tissue and the serum levels of lipopolysaccharide (LPS) and D-lactic acid were determined. The gut microbiota composition and the fecal contents of short-chain fatty acids were detected. The expression levels of genes related to the intestinal barrier and the nuclear factor kappa-B (NF-kB) pathway were determined. Results   The high dose of Bifidobacterium animalis subsp. lactis XLTG11 significantly increased the body mass gain and anti-inflammatory cytokine levels (P < 0.05), and significantly decreased cecum mass, fecal water content, fecal consistency score and proinflammatory cytokine levels in the mouse model mice of AAD. Moreover, it significantly up-regulated the gene expression levels of ZO-1, occludin, claudin-1 and MUC2, regulated the composition of the gut microbiota, evidently increased the fecal contents of acetate, propanoate, and butanoate, and significantly down-regulated the expression levels of genes related to the Toll like receptor 4 (TLR4), myeloid differentiation factor (MYD88) and NF-κB signaling pathway. Conclusion   Bifidobacterium animalis subsp. lactis XLTG11 can effectively alleviate AAD symptoms in mice by regulating cytokines and the gut microbiota, increasing fecal short-chain fatty acid contents, increasing the expression levels of intestinal barrier related genes and inhibiting the activation of the TLR4/MyD88/NF-kB signaling pathway.}
}