@article{Liu2025, 
author = {Qingqing Liu and Lingling Guo and Liqiang Liu and Chuanlai Xu and Hua Kuang and Aihua Qu},
title = {Quantitative assessment of pyriproxyfen in various matrices using a colloidal gold immunochromatographic sensor},
year = {2025},
journal = {Nano Research},
volume = {18},
number = {12},
pages = {94907995},
keywords = {environmental monitoring, food safety, pyriproxyfen, colloidal gold immunochromatographic test strips},
url = {https://www.sciopen.com/article/10.26599/NR.2025.94907995},
doi = {10.26599/NR.2025.94907995},
abstract = {The widespread application of pyriproxyfen (PPF) in agriculture has led to its accumulation in environmental and food samples, posing potential risks to environmental safety and human health. Therefore, a reliable and sensitive detection method is urgently needed. In this study, suitable PPF hapten was designed using computer analysis. A highly specific monoclonal antibody (mAb) against PPF was successfully developed, exhibiting a half-maximal inhibitory concentration of 1.55 ng/mL. Based on this antibody, a colloidal gold immune test strip was established for the on-site detection of PPF in lake water, soil, strawberries, and cabbage, achieving calculated limits of detection (cLOD) of 0.76, 1.87, 5.25, and 4.35 ng/mL, respectively. The spiked recovery rates ranged from 91.7% to 110.8%, with coefficients of variation between 2.17% to 8.71%. The detection results were consistent with those obtained using instrumental method, confirming that the developed colloidal gold immunochromatographic test strip (CGITS) enables accurate, rapid, and on-site detection of PPF in environmental and food samples.}
}