@article{Chen2025, 
author = {Yuting Chen and Haoyang Han and Lei Liu and Junxu Yang and Hao Wang and Ying Tan and Feiying Yin and Jianwen Cheng and Li Zheng and Jinmin Zhao},
title = {3D bioprinted nanozyme-enhanced GelMA hydrogel with antioxidant/anti-inflammatory potential for bone repair},
year = {2025},
journal = {Nano Research},
volume = {18},
number = {10},
pages = {94907979},
keywords = {photothermal performance, Nrf2 pathway, three-dimensional (3D) bioprinting, Mn3O4 nanozyme, bone marrow mesenchymal stem cells (BMSCs)},
url = {https://www.sciopen.com/article/10.26599/NR.2025.94907979},
doi = {10.26599/NR.2025.94907979},
abstract = {The repair of large-scale bone defects is still a challenge in clinical orthopedics. Especially, excessive reactive oxygen species (ROS)-induced oxidative stress injury greatly affected bone healing. In this study, we innovatively developed an antioxidant three-dimensional (3D)-bioprinted M-Mn3O4@Gel by integrating M-Mn3O4 nanozyme into photo-crosslinked gelatin methacryloyl (GelMA) for the therapy of bone defects. Results showed that the incorporation of M-Mn3O4 not only enhanced the mechanical properties of the nanocomposite hydrogel with the compressive modulus 141.79% higher than that of pure GelMA, but also maintained excellent 3D printability. In vitro studies confirmed that the 3D-printed M-Mn3O4@Gel exhibited favorable biocompatibility and cell adhesion. It significantly reduced oxidative stress through efficient ROS scavenging, restored mitochondrial function, and ultimately demonstrated remarkable osteogenic capacity, highlighting the efficacy of control-released nanozymes. More importantly, under near-infrared (NIR) irradiation, M-Mn3O4@Gel demonstrated further enhanced ROS-scavenging capacity and bone regeneration potential. Mechanistically, M-Mn3O4@Gel promoted osteogenesis by upregulating heat shock protein 40 kDa (HSP40) and HSP70 expression, effectively mitigating the overactivation of the Nrf2 pathway. This study innovatively combines nanozyme technology with 3D-printed hydrogel materials, offering a novel strategy to address the challenge of oxidative stress in bone regeneration.}
}