TY  - JOUR
AU  - YAO, Xiaolong
AU  - HAN, Lei
AU  - LIU, Xudong
AU  - LUO, Yue
AU  - WU, Qiong
AU  - AN, Huaming
AU  - WU, Xiaomao
PY  - 2022
TI  - Simultaneous Determination of 11 Pesticides in Rosa roxburghii by QuEChERS-Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
JO  - Food Science
SN  - 1002-6630
SP  - 309
EP  - 316
VL  - 43
IS  - 16
AB  - ObjectiveA method for the determination of 11 common pesticides in Rosa roxburghii Tratt. was established using QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).MethodsThe QuEChERS conditions were optimized by performing spiked recovery tests. Acetonitrile was used for extraction, and primary secondary amine (PSA) and graphitized carbon black (GCB) were used for purification. The chromatographic separation was achieved using gradient elution with a mobile phase consisting of 0.1% formic acid water and acetonitrile at a flow rate of 0.4 mL/min. In the positive ion mode using an electrospray ionization source, the fragmentor voltage, collision energy and ion pairs for each pesticide were optimized to obtain the best response, and the pesticides were quantified by matrix-matched calibration.ResultsThe limits of detection (LODs) for all pesticides were in the range of 1.31–8.56 μg/kg, and the limits of quantitation (LOQs) ranged from 4.13–43.57 μg/kg. The correlation coefficients (R) of the calibration curves were in the range of 0.9912–0.9999. The average recoveries at three spiked levels ranged from 84.5% to 103.8% (n = 6) with relative standard deviations (RSDs) of 1.2%–8.5%.ConclusionThis method was rapid, sensitive and precise, and could provide technical support for the determination of pesticide residues in R. roxburghii.
UR  - https://doi.org/10.7506/spkx1002-6630-20210629-339
DO  - 10.7506/spkx1002-6630-20210629-339