TY  - JOUR
AU  - ZHAO, Jiani
AU  - CHEN, Hong
AU  - CHEN, Yinge
AU  - WENG, Ling
AU  - ZHANG, Lingjing
AU  - ZHANG, Zhigang
AU  - LIN, Jiashi
AU  - CAO, Minjie
PY  - 2022
TI  - Purification and Characterization of Dipeptidyl Peptidase-Ⅳ from Bovine Kidney
JO  - Food Science
SN  - 1002-6630
SP  - 194
EP  - 200
VL  - 43
IS  - 16
AB  - The purpose of this study is to efficiently prepare high-purity dipeptidyl peptidase-Ⅳ (DPP-Ⅳ) from beef kidney by sequential acid precipitation, ammonium sulfate fractionation and gel filtration chromatography. Under reducing conditions, the molecular mass of the purified protein was estimated to be 106 kDa with a purification fold of 169.9 and a yield of 9.5%. Twelve peptides with a total of 517 amino acid residues were identified from the DPP-Ⅳ by mass spectrometry. The matching degree of the 12 peptides with the amino acid sequence of bovine DPP-Ⅳ standard was 100%. The DPP-Ⅳ was found to be a dimeric glycoprotein (210 kDa), with an isoelectric point of 5.8. The secondary structure of the DPP-Ⅳ had a typical β-sheet conformation, and the thermal denaturation temperature was (72.7 ± 0.3) ℃. Metal ions such as Zn2+, Fe2+ and Fe3+ revealed a significant inhibitory effect on the activity of DPP-Ⅳ. The inhibition rate of 1 mmol/L Zn2+ on the enzyme’s activity was as high as 98%.
UR  - https://doi.org/10.7506/spkx1002-6630-20210805-069
DO  - 10.7506/spkx1002-6630-20210805-069