@article{NIU2022, 
author = {Pilian NIU and Xuezhang ZHOU and Mingsheng BAI},
title = {Influence of Chlorogenic Acid from Lycium barbarum on Cardiac Fibroblast Fibrosis Induced by Transforming Growth Factor-β1},
year = {2022},
journal = {Food Science},
volume = {43},
number = {7},
pages = {158-164},
keywords = {myocardial fibrosis, transforming growth factor (TGF)-β1, TGF-β1/Smads signaling pathway, chlorogenic acid, cardiac fibroblasts},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20210103-008},
doi = {10.7506/spkx1002-6630-20210103-008},
abstract = {Objective   To investigate the protective effect of chlorogenic acid from Lycium barbarum on cardiac fibroblast (CF) fibrosis induced by transforming growth factor-β1 (TGF-β1).          Methods   A TGF-β1 concentration of 10 ng/mL was used to induce CF fibrosis. The CFs were further treated with different concentrations of chlorogenic acid, and their proliferation was detected by cell counting kit-8 (CCK-8) assay. The expression of the fibrosis markers collagen (Col) Ⅰ and Col Ⅲ was detected by enzyme linked immunosorbent assay (ELISA) and α-smooth muscle actin (α-SMA) by immunofluorescence. The mRNA expression levels of Acta2, Twist1, Snail1, Snail2, Smad4 and S100A4 were detected by quantitative real-time polymerase chain reaction (qPCR). Western blot analysis was used to evaluate the expression levels TGF-β1/Smads signaling pathway-related proteins and their phosphorylation levels.           Results   Chlorogenic acid from Lycium barbarum could inhibit abnormal cell proliferation, and down-regulate the expression of Col Ⅰ, Col Ⅲ and α-SMA, the mRNA expression of Acta2, Twist1, Snail1, Snail2, Smad4 and S100A4 (P < 0.001) and the expression of the TGF-β1/Smads signaling pathway-related proteins (P < 0.001).           Conclusion   Within the concentration range tested in this study, chlorogenic acid from Lycium barbarum inhibits the fibrosis process of CFs induced by TGF-β1, and the mechanism may be related to the TGF-β1/Smads signaling pathway.}
}