TY  - JOUR
AU  - MA, Zhihua
AU  - NIU, Pilian
AU  - XU, Xingbo
AU  - ZHANG, Ziping
AU  - ZHOU, Xuezhang
AU  - BAI, Mingsheng
PY  - 2022
TI  - Inhibitory Mechanism of Lycium barbarum Polysaccharide against Cardiac Fibroblast Activation Induced by TGF-β1
JO  - Food Science
SN  - 1002-6630
SP  - 144
EP  - 150
VL  - 43
IS  - 19
AB  - Objective   To explore the ameliorative effect of Lycium barbarum polysaccharide (LBP) on TGF-β1-induced cardiac fibrosis in cardiac fibroblasts (CFs). Methods   Mouse primary CFs were cultured and treated with 10 ng/mL TGF-β1 to induce fibrosis. After treatment with LBP, the morphology of cells was observed by microscopy. The expression of α-smooth muscle actin (α-SMA) was detected by immunofluorescence. Western blot was used to detect the expression and phosphorylation levels of proteins involved in the TGF-β1/Smads signaling pathway. The mRNA expression levels of Snail, Twist and S100A4 were detected by real-time quantitative polymerase chain reaction. Results   Compared with the control group (spindle-like in shape), the morphology of TGF-β1-induced cells was longer and narrower, and the number of cells increased significantly. The relative expression level of α-SMA, and the expression and phosphorylation levels of proteins involved in the TGF-β1/Smads signaling pathway increased, and the mRNA expression of Snail, Twist and S100A4 was significantly elevated (P  < 0.01). After treatment with LBP, the proliferation of CFs induced by TGF-β1 was significantly inhibited (P  < 0.001), the expression of α-SMA and proteins involved in the TGF-β1/Smads signaling pathway in CFs decreased, and the mRNA expression of Snail, Twist and S100A4 was down-regulated (P  < 0.001). Conclusion   Lycium barbarum polysaccharide can ameliorate TGF-β1-induced cardiac fibrosis, in CFs and its mechanism may be related to the inhibition of the TGF-β1/Smads signaling pathway.
UR  - https://doi.org/10.7506/spkx1002-6630-20210927-323
DO  - 10.7506/spkx1002-6630-20210927-323