TY  - JOUR
AU  - YIN, Jian
AU  - WU, Ruiyun
AU  - HU, Jinrong
AU  - LI, Pinglan
PY  - 2022
TI  - Purification and Identification of Dipeptidyl Peptidase Ⅳ Inhibitory Peptide from Sturgeon Skin Collagen
JO  - Food Science
SN  - 1002-6630
SP  - 195
EP  - 203
VL  - 43
IS  - 6
AB  - In order to prepare dipeptidyl peptidase Ⅳ (DPP-Ⅳ) inhibitory peptide, sturgeon skin collagen was hydrolyzed by protease. A combination of one-factor-at-a-time method and response surface methodology was used optimize the preparation conditions based on percentage of DPP-Ⅳ inhibition. The optimal enzymatic hydrolysis conditions were determined as follows: temperature 50 ℃, solid-to-liquid ratio 1:100 (g/mL), pH 6.12, enzyme dosage 10170.35 U/g, and enzymatic hydrolysis time 12.12 h. The hydrolysate prepared under the optimized conditions was separated and purified by sequential ultrafiltration, gel filtration chromatography and reverse-phase high performance liquid chromatography (RP-HPLC). As identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), the amino acid composition of the purified peptide with DPP-Ⅳ inhibitory activity was GPSGLDGAK, and its half-maximum inhibitory concentration (IC50) value was (61.27 ± 1.16) μmol/L. The results of this study can provide a reference for the production of new bioactive components from sturgeon skin.
UR  - https://doi.org/10.7506/spkx1002-6630-20210131-374
DO  - 10.7506/spkx1002-6630-20210131-374