@article{HU2022, 
author = {Chaoqiong HU and Lijun WANG and Xuan TAO and Yun ZHU and Yu LIANG and Xiao YANG and Xianggui CHEN and Yukun HUANG},
title = {Development and Application of Broad-Spectrum Aptamer-Magnetic Separation Assisted Fluorescence Method for Determination of Organophosphorus Pesticide Residues in ‘Shatangju’ Mandarine},
year = {2022},
journal = {Food Science},
volume = {43},
number = {4},
pages = {264-269},
keywords = {aptamer, nanomagnetic beads, organophosphorus pesticides, fluorescence analysis, ‘Shatangju’ mandarin},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20201114-156},
doi = {10.7506/spkx1002-6630-20201114-156},
abstract = {In this paper, a magnetic separation assisted fluorescence method for the determination of three common organophosphorus pesticides (OPPs) in ‘Shatangju’ mandarin was established. Magnetic beads were synthesized by solvothermal method and modified by polypyrrole (PPy) to form Fe3O4@PPy nanoparticles as a fluorescence quenching agent. This method was based on the use of a 6-carboxyfluorescein (FAM)-labeled aptamer (S4-29) by virtue of its broad-spectrum recognition ability for OPPs. When Fe3O4/PPy nanoparticles mass ratio, Fe3O4@PPy nanoparticles concentration and incubation time were 1:1.5, 0.2 mg/mL, and 120 min, respectivley, the detection limits of phorate, omethoate and profenofos were 4.17 × 10-4, 2.77 × 10-4, and 4.67 × 10-4 μg/kg, and the recoveries ranged from 79.94% to 108.00% with relative standard deviations (RSDs) between 3.05% and 7.39%. In conclusion, this method has strong selectivity and high accuracy and can be used for the analysis of banned OPPs in agricultural products.}
}