@article{GUO2022, 
author = {Tianrong GUO and Wenlin WU and Yin ZHANG and Yuping WAN and Mei YE and Daiwei CHEN and Xia HUANG and Longyi ZHANG},
title = {Multi-objective Rapid Screening of 30 Protein Assimilation Hormones and Glucocorticoids in Fish by Ultra-high Performance Liquid Chromatography Coupled to Quadrupole/Orbitrap High-Resolution Mass Spectrometry},
year = {2022},
journal = {Food Science},
volume = {43},
number = {4},
pages = {321-330},
keywords = {ultra-high performance liquid chromatography coupled to quadrupole/orbitrap high-resolution mass spectrometry, fish, protein assimilation hormone, glucocorticoid, multi-target rapid screening},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20210203-047},
doi = {10.7506/spkx1002-6630-20210203-047},
abstract = {A method for rapid screening and identification of 30 protein assimilation hormones and glucocorticoids in fish was established using ultra-high performance liquid chromatography coupled to quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). The samples were extracted with 80% acetonitrile aqueous solution (containing 0.2% formic acid), centrifuged, purified by solid phase extraction (SPE) on Oasis PRiME HLB column, and dried with nitrogen blow. The residue was re-dissolved. The chromatographic separation was performed on a Waters Acquity BEH C18 (2.1 mm × 100 mm, 1.7 μm) column through gradient elution using a mobile phase consisting of 20 mmol/L ammonium acetate aqueous solution containing 0.1% formic acid (A) and acetonitrile (B). The target analytes were detected in the positive and negative ion mode using a heated electrospray ionization (HESI) source through primary full scan/data-dependent secondary scan (full MS/dd-MS2) monitoring, and quantitated by matrix-matched external standard method. The results showed that good linearity was observed for the 30 hormones in the concentration range of 0.5–100 ng/mL with correlation coefficients (r) greater than 0.9950. The limits of detection were between 0.2 and 1.0 μg/kg, and the limits of quantification were between 0.5 and 2.0 μg/kg. The recoveries were 69.7%–103.2% with relative standard deviations (RSDs) of 2.3%–9.4% at three spiked levels for four different fishes (turbot, mandarin fish, mullet and grass carp). The proposed method is efficient, accurate and reliable, and is suitable for the rapid multi-objective screening and quantitative analysis of protein assimilation hormones and glucocorticoids in fish.}
}