TY  - JOUR
AU  - YANG, Huixuan
AU  - LUO, Xin
AU  - ZHU, Lixian
AU  - YANG, Xiaoyin
AU  - HAN, Guangxing
AU  - LI, He
AU  - DONG, Pengcheng
AU  - ZHANG, Yimin
PY  - 2022
TI  - Isolation, Identification and Biological Characteristics of Bioprotective Bacteria from Vacuum Packaged Chilled Beef
JO  - Food Science
SN  - 1002-6630
SP  - 151
EP  - 158
VL  - 43
IS  - 22
AB  - A total of seven lactic acid bacteria strains with bioprotective potential were isolated and characterized from vacuum-packaged chilled beef. By morphological observation, physiological and biochemical identification and 16S rDNA sequencing, they were determined as Lactobacillus sakei. All isolates grew well at 25‒35 ℃, and RS-16 and RS-25 grew better and had a stronger acid-producing ability. Antibiotic resistance and virulence genes were detected in RS-33 and RS-41 but not any of the other strains. The results of agar diffusion test showed that the seven isolates had antagonistic effect on all indicator strains, and the antagonistic effect of strains RS-16 and RS-25 on Salmonella typhimurium was better than that of the other strains (P < 0.05). Organic acids secreted by the isolated strains were found to have antibacterial activity.
UR  - https://doi.org/10.7506/spkx1002-6630-20211208-094
DO  - 10.7506/spkx1002-6630-20211208-094