@article{SU2022, 
author = {Anxiang SU and Ye HU and Qiuhui HU and Hui XU and Jianhui LIU and Minhao XIE and Fei PEI and Wenjian YANG},
title = {Inhibitory Effect of Flammulina velutipes Proteoglycan on Lipopolysaccharide-induced Inflammation in Caco-2/RAW264.7 Co-culture Model},
year = {2022},
journal = {Food Science},
volume = {43},
number = {3},
pages = {146-151},
keywords = {inflammation, lipopolysaccharide, Caco-2 cells, proteoglycan, Flammulina velutipes},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20210118-194},
doi = {10.7506/spkx1002-6630-20210118-194},
abstract = {With the accelerating pace of life and the changing lifestyle, the incidence of inflammatory bowel disease is increasing. Finding inflammation-inhibitory components from the diet is an effective way to prevent and control inflammatory bowel disease. In this study, the inhibitory effect of a proteoglycan extracted from Flammulina velutipes proteoglycans (FPG1-1) on lipopolysaccharide (LPS)-induced inflammation in a co-culture model of Caco-2 intestinal epithelial cells and macrophages RAW264.7 was evaluated by measuring nitric oxide (NO), reactive oxygen species (ROS) and cytokines levels. The results showed that the FPG1-1 at a concentration of 200 μg/mL extremely significantly reduced the production of NO and ROS in macrophages in comparison with the model group (P < 0.01). Besides, FPG1-1 up-regulated the secretion of interleukin (IL)-10 and down-regulated the secretion of tumor necrosis factor (TNF)-α, IL-1β and IL-6. The above results indicate that FPG1-1 can effectively inhibit lipopolysaccharide-induced inflammation in the co-culture model of intestinal epithelial cells and macrophages. This research provides a theoretical basis for the utilization of functional ingredients from Flammulina velutipes and the development of functional foods against inflammatory bowel disease.}
}