@article{HUAN2025, 
author = {Jiaxin HUAN and Yanping HUANG and Yanzhe NIU and Hong SONG and Manshun LIU and Chunlong YUAN},
title = {Efficient Degradation of Ochratoxin A by Zn-Metal-Organic Framework Immobilized Lipase},
year = {2025},
journal = {Food Science},
volume = {46},
number = {16},
pages = {175-182},
keywords = {immobilization, metal-organic framework, Amano lipase A, ochratoxin A degradation},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20241227-237},
doi = {10.7506/spkx1002-6630-20241227-237},
abstract = {To enhance the stability of Amano lipase A (ANL) and its degradation efficiency toward ochratoxin A (OTA), ANL was immobilized on a Zn-metal-organic framework (Zn-MOF) carrier by a one-step in situ method, yielding ANL@Zn-MOF. Based on OTA degradation rate, the optimal immobilization conditions were determined as 8 mg, 2 h and 400 r/min for enzyme loading, immobilization time, and stirring speed, respectively. ANL@Zn-MOF was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The results confirmed the successful loading of ANL onto Zn-MOF. ANL@Zn-MOF exhibited superior alkali tolerance, thermal stability, and ionic strength stability compared with free ANL. ANL@Zn-MOF degraded OTA more rapidly. Specifically, it (containing 20 ng/mL ANL) completely degraded OTA in 8 h, which was shortened by 66.7% compared with free ANL. Moreover, in a 500 mL reaction system, ANL@Zn-MOF degraded 69.3% of OTA in 12 h. Additionally, ANL@Zn-MOF exhibited excellent reusability, retaining 78% of its activity after five cycles of reuse. This study establishes a foundation for the application of ANL@Zn-MOF in degrading OTA in food systems and provides a reference for the use of MOFs in enzyme immobilization.}
}