@article{Zheng2025, 
author = {Zeyang Zheng and Feibai Zhou and Xiaoling Liu and Mouming Zhao},
title = {Formation of oyster peptide-stabilized selenium nanoparticles and their hepatoprotective effect against hydrogen peroxide-induced cytotoxicity in HepG2 cells},
year = {2025},
journal = {Food Science of Animal Products},
volume = {3},
number = {4},
pages = {9240142},
keywords = {oxidative stress, hepatoprotective effect, selenium nanoparticle, oytster peptides, HepG2 cell model},
url = {https://www.sciopen.com/article/10.26599/FSAP.2025.9240142},
doi = {10.26599/FSAP.2025.9240142},
abstract = {In the present study, a new type of selenium nanoparticles (SeNPs) was synthesized using oyster protein hydrolysates (OPH) as both a stabilizer and capping agent upon ultrasonication. OPH with alcohol dehydrogenase activation activity was prepared and used to fabricate OPH-SeNPs, where monodisperse SeNPs with a transparent orange appearance, ranging from 140 to 310 nm, were obtained. Physicochemical analysis further suggested a weak interaction between SeNPs and the –NH, C=O, COO–, and C–N groups of the OPH, and the formed nanocomposite with improved stability against aggregation was in an amorphous state. The hepatoprotective effect of OPH-SeNPs on HepG2 cells showed that pre-incubation with OPH-SeNPs significantly decreased cell apoptosis induced by hydrogen peroxide and could well maintain cell integrity. A reduction in intracellular reactive oxygen species was found, along with ameliorated activity of internal antioxidant enzymes. The observed upregulation of key antioxidant-related components, i.e., glutathione peroxidase, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1, suggests activation of the Nrf2-antioxidant response element signaling pathway, which mechanistically explains the hepatoprotective effect of OPH-SeNPs against hydrogen peroxide-induced cytotoxicity in HepG2 cells.}
}