@article{WEI2025, 
author = {Zijie WEI and Zhihua TAO and Jingyu LI and Junhao ZHANG and Kegang WU and Xianghua CHAI and Hongpeng YU and Dong HE and Xiaoli LIU and Xuejuan DUAN and Pingping WANG},
title = {Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans},
year = {2025},
journal = {Food Science},
volume = {46},
number = {13},
pages = {205-213},
keywords = {oak essential oil, pancreatic lipase, Caenorhabditis elegans, lipid metabolism, gene},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20241225-217},
doi = {10.7506/spkx1002-6630-20241225-217},
abstract = {This study investigated the lipid-lowering activity and action mechanism of oak essential oil (OEO). OEO had strong in vitro lipid-lowering activity, and its half-maximal inhibitory concentration (IC50) on pancreatic lipase, cholesterol esterase and α-glucosidase were (0.62 ± 0.05), (0.20 ± 0.04) and (0.63 ± 0.01) mg/mL, respectively. Enzymatic kinetics and fluorescence emission spectroscopy showed that the inhibition type of OEO on pancreatic lipase was reversible non-competitive inhibition, and the quenching type was static quenching. The in vivo lipid-lowering activity of OEO was also assessed using Caenorhabditis elegans as a model organism. It turned out that OEO increased lipid droplets with a diameter of 0–0.6 μm and decreased lipid droplets with a diameter of 1.2–1.8 μm in high-fat C. elegans; 20 μg/mL of OEO reduced the fat content and total triglyceride level of high-fat C. elegans by 26.46% and 21.35%, respectively. The results of real-time polymerase chain reaction (PCR) showed that OEO significantly up-regulated the relative expression levels of lipid hydrolysis genes (atgl-1 and hosl-1) and fatty acid beta oxidation genes (acs-2), and significantly down-regulated the relative expression levels of fat synthesis genes (fat-5, fat-6, fat-7 and sbp-1). This study indicates that OEO has lipid-lowering activity, providing data support for its application for the alleviation of obesity.}
}