@article{ZHAI2023, 
author = {Hongwen ZHAI and Hongyu MA and Junmei MA and Meirong CAO and Jing WANG and Juan WANG and Qiang LI},
title = {Simultaneous Determination of Vitamins A, D and E in Foods for Special Medical Purposes by Online Solid Phase Extraction Combined with Two-Dimensional Liquid Chromatography},
year = {2023},
journal = {Food Science},
volume = {44},
number = {20},
pages = {315-320},
keywords = {two-dimensional liquid chromatography, online solid phase extraction, food for special medical purposes, VA, VD, VE},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20220923-241},
doi = {10.7506/spkx1002-6630-20220923-241},
abstract = {A rapid method for the determination of VA, VD (VD2, VD3) and VE (α-, β-, γ-, and δ-vitamin E) in foods for special medical purposes was established using online solid phase extraction (SPE) combined with two-dimensional liquid chromatography (2D-LC). After saponification, enrichment and purification of VA, VD and VE from samples were accomplished on an online SPE cartridge. VA and four VEs were quantitatively analyzed by the first-dimensional LC, and VD was transferred by heart-cutting to the second-dimensional chromatographic system for separation and detection. SPE columns with different fillings, sample loading solvents, and eluent composition were evaluated, and 2D-LC conditions were systematically optimized. Good linearity was achieved for all vitamins tested with correlation coefficients (R2) greater than 0.997. The mean recoveries of the developed method were 84.4%–109.0% with relative standard deviation (RSD) of 0.34%–6.55% (n = 6). This method was highly automated with low detection errors and high detection efficiency, and could be used for the analysis of vitamins in foods for special medical purposes.}
}