@article{Peng2025, 
author = {Yanxi Peng and Honggen Yuan and Zhanjie Jiang and Xiaoqing Ou and Qian Zhang and Kexin Yi and Yanbin Meng and Qun Xie},
title = {N6-Methyladenosine Promotes the Transcription of c-Src Kinase via IRF1 to Facilitate the Proliferation of Liver Cancer},
year = {2025},
journal = {Oncology Research},
volume = {33},
number = {7},
pages = {1679-1693},
keywords = {N6-methyladenosine (m6A), c-Src, interferon regulatory factor 1 (IRF1), liver cancer, hepatocellular carcinoma (HCC)},
url = {https://www.sciopen.com/article/10.32604/or.2025.062747},
doi = {10.32604/or.2025.062747},
abstract = {BackgroundExpression of mRNA is widely regulated by N6-methyladenosine (m6A). An increasing number of studies have shown that m6A methylation, facilitated by methyltransferase 3 (METTL3), is crucial in the progression of tumors. Previous reports have indicated the involvement of both METTL3 and c-Src kinase in the evolution of liver cancer. However, the potential connection between c-Src and the METTL3-mediated mechanism in liver cancer progression remains elusive.MethodsThe correlation expression between c-Src and METTL3 between liver cancer patients and the control group was analyzed using the TCGA database, and was further demonstrated by Western blot and RT-qPCR. The functional roles of c-Src in METTL3-regulated liver cancer progression were investigated by cell proliferation assays and colony formation assays. The regulatory mechanism of METTL3 in c-Src expression was accessed by RNA-immunoprecipitation (RIP)-qPCR.ResultsWe demonstrated that c-Src kinase promoted liver cancer development, and the expression of SRC (encodes c-Src kinase) was positively correlated with METTL3 in liver cancer cases. We showed that SRC mRNA could be m6A-modified, and METTL3 regulated the transcription of SRC mRNA through interferon regulatory factor 1 (IRF1). We revealed that IRF1, the expression of which was positively regulated by METTL3, was a novel transcription factor of c-Src. Lastly, The pro-proliferative effect of METTL3 on hepatocellular carcinoma was mechanistically linked to IRF1/c-Src axis activation, as evidenced by our experimental data.ConclusionResults suggested that the METTL3/IRF1/c-Src axis played potential oncogenic roles in liver cancer development and the axis may be a promising therapeutic target in the disease.}
}