@article{Zhu2025, 
author = {Shichao Zhu and Jing Liu and Xuewen Qiu and Guoqing Cai and Mei Gao},
title = {TGF-β signaling in organoids: The mechanisms and applications in 3D},
year = {2025},
journal = {Cell Organoid},
volume = {1},
number = {2},
pages = {9410015},
keywords = {stem cells, regenerative medicine, fibrosis, organoids, disease modeling, TGF-β signaling, tissue homeostasis},
url = {https://www.sciopen.com/article/10.26599/CO.2025.9410015},
doi = {10.26599/CO.2025.9410015},
abstract = {The Transforming Growth Factor-beta (TGF-β) signaling pathway is a master regulator of cellular processes, including proliferation, differentiation and apoptosis, with profound implications for tissue homeostasis and disease pathogenesis. This review delves into the mechanisms and applications of TGF-β signaling within organoids, three-dimensional (3D) cultures that mimic human tissue architecture and function. We systematically explore the multifaceted role of TGF-β in organoid initiation, growth, and differentiation, highlighting its influence on stem cell self-renewal and lineage specification. The interplay between TGF-β and other signaling pathways, such as Wnt/β-catenin and Notch, is critical for maintaining tissue homeostasis and regulating stem cell niches. Furthermore, we discuss the significance of TGF-β signaling in modeling diseases like cancer and fibrosis, where its dysregulation is often implicated. Modulating TGF-β signaling in organoids holds vast therapeutic potential, offering insights into disease pathogenesis, predicting drug responses, and developing personalized treatment strategies. As our understanding of TGF-β signaling and organoid technology advances, these systems hold promise for groundbreaking biomedical research, potentially revolutionizing regenerative medicine and personalized therapeutics.}
}