TY  - JOUR
AU  - Fan, Guiyan
AU  - Duan, Shaoguang
AU  - Yang, Yuting
AU  - Duan, Yanfeng
AU  - Jian, Yinqiao
AU  - Hu, Jun
AU  - Liu, Zhiyuan
AU  - Guo, Yang-dong
AU  - Jin, Liping
AU  - Xu, Jianfei
AU  - Li, Guangcun
PY  - 2025
TI  - Fine-mapping and candidate gene analysis of tuber eye depth in potato
JO  - Horticultural Plant Journal
SN  - 2095-9885
SP  - 1248
EP  - 1259
VL  - 11
IS  - 3
AB  - Eye depth is an important agronomic trait affecting tubers' appearance, quality, and processing suitability. Hence, cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding. In this study, based on the primary mapping of the tuber eye-depth locus using a small primary-segregating population, a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further. A major quantitative trait locus for eye-depth on chromosome 10 was identified (designated qEyd10.1) using BSA-seq and traditional QTL mapping methods. The qEyd10.1 could explain 55.0% of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis. To predict candidate genes, tissue sectioning and RNA-seq of the specific tuber tissues were performed. Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates. These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.
UR  - https://doi.org/10.1016/j.hpj.2024.03.006
DO  - 10.1016/j.hpj.2024.03.006