@article{DAI2024, 
author = {Yongjin DAI and Jing SUN and Moran ZHANG and Yujuan LIU and Hongzhou CHEN and Yingjian LU},
title = {Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms},
year = {2024},
journal = {Food Science},
volume = {45},
number = {22},
pages = {351-360},
keywords = {clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins systems, trans-cleavage, foodborne pathogen, nucleic acid detection},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20231205-029},
doi = {10.7506/spkx1002-6630-20231205-029},
abstract = {Foodborne pathogenic microorganisms are a serious threat to food safety. Currently, traditional biochemical assays are facing a series of challenges in the detection of foodborne pathogens. In recent years, with the gradual development of CRISPR (clustered regularly interspaced short palindromic repeats) technology, the CRISPR/CRISPR-associated proteins (Cas) system with trans-cleavage activity has received much attention from researchers. In this article, the origin, classification and principles of CRISPR/Cas systems with trans-cleavage activity are summarized. This paper also reviews the versatile application of trans-cleavage CRISPR/Cas systems in the field of foodborne pathogens, and concludes with a discussion of the advantages, disadvantages and future directions of this technology.}
}