@article{Zeng2025, 
author = {Jianqi Zeng and Dehui Zhao and Li Yang and Yufeng Yang and Dan Liu and Yubing Tian and Fengju Wang and Shuanghe Cao and Xianchun Xia and Zhonghu He and Yong Zhang},
title = {Fine mapping and candidate gene analysis of a major QTL for grain length on chromosome 5BS in bread wheat},
year = {2025},
journal = {Journal of Integrative Agriculture (JIA)},
volume = {24},
number = {7},
pages = {2465-2474},
keywords = {breeding-friendly marker, candidate gene prediction, fine mapping, grain length, Triticum aestivum L.},
url = {https://www.sciopen.com/article/10.1016/j.jia.2024.04.033},
doi = {10.1016/j.jia.2024.04.033},
abstract = {Large grain is a favorable trait for appearance quality and a large sink potential in wheat breeding. The stable QTL QGl.caas-5BS for grain length was previously identified in a recombinant inbred line population from the cross of Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895). Here, a BC1F6 residual heterozygous line was selected from the cross of a ZM871/ZM895//ZM871 population, and six heterozygous recombinant plants were identified in the BC1F7 population from self-pollination of the heterozygous line. QGl. caas-5BS was delimited into an interval of approximately 2.2 Mb flanked by markers Kasp_5B33 and Kasp_5B2 (25.3–27.5 Mb) by phenotyping and genotyping the secondary mapping populations derived from these heterozygous recombinant plants. Five genes were predicted as candidates of QGl.caas-5BS based on sequence polymorphism and differential expression analyses. Further mutation analysis showed that TraesCS5B02G026800 is likely the causal gene of QGl.caas-5BS. The gene-specific marker Kasp_5B_Gl for TraesCS5B02G026800 was developed, and a significant genetic effect of QGl.caas-5BS on grain length was identified in a validation population of 166 cultivars using this marker. These findings lay a good foundation for map-based cloning of QGl.caas-5BS and provide a breeding-applicable marker for the improvement of grain length in wheat.}
}