TY  - JOUR
AU  - Zeng, Jianqi
AU  - Zhao, Dehui
AU  - Yang, Li
AU  - Yang, Yufeng
AU  - Liu, Dan
AU  - Tian, Yubing
AU  - Wang, Fengju
AU  - Cao, Shuanghe
AU  - Xia, Xianchun
AU  - He, Zhonghu
AU  - Zhang, Yong
PY  - 2025
TI  - Fine mapping and candidate gene analysis of a major QTL for grain length on chromosome 5BS in bread wheat
JO  - Journal of Integrative Agriculture (JIA)
SN  - 2095-3119
SP  - 2465
EP  - 2474
VL  - 24
IS  - 7
AB  - Large grain is a favorable trait for appearance quality and a large sink potential in wheat breeding. The stable QTL QGl.caas-5BS for grain length was previously identified in a recombinant inbred line population from the cross of Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895). Here, a BC1F6 residual heterozygous line was selected from the cross of a ZM871/ZM895//ZM871 population, and six heterozygous recombinant plants were identified in the BC1F7 population from self-pollination of the heterozygous line. QGl. caas-5BS was delimited into an interval of approximately 2.2 Mb flanked by markers Kasp_5B33 and Kasp_5B2 (25.3–27.5 Mb) by phenotyping and genotyping the secondary mapping populations derived from these heterozygous recombinant plants. Five genes were predicted as candidates of QGl.caas-5BS based on sequence polymorphism and differential expression analyses. Further mutation analysis showed that TraesCS5B02G026800 is likely the causal gene of QGl.caas-5BS. The gene-specific marker Kasp_5B_Gl for TraesCS5B02G026800 was developed, and a significant genetic effect of QGl.caas-5BS on grain length was identified in a validation population of 166 cultivars using this marker. These findings lay a good foundation for map-based cloning of QGl.caas-5BS and provide a breeding-applicable marker for the improvement of grain length in wheat.
UR  - https://doi.org/10.1016/j.jia.2024.04.033
DO  - 10.1016/j.jia.2024.04.033