@article{LIU2025, 
author = {Mingming LIU and Haiyan YAN and Zhaoxu ZHOU and Yuhua YE and Zhigang LI and Zhaojing XIAO},
title = {Rapid Detection of Dog-Derived Ingredients by Real-Time Recombinase-Aided Amplification},
year = {2025},
journal = {Meat Research},
volume = {39},
number = {4},
pages = {24-29},
keywords = {real-time recombinase-aided amplification, dog-derived ingredients, meat adulteration},
url = {https://www.sciopen.com/article/10.7506/rlyj1001-8123-20241009-261},
doi = {10.7506/rlyj1001-8123-20241009-261},
abstract = {In order to establish a real-time recombinase aided amplification (Rt-RAA) method to rapidly detect dog-derived ingredients, three pairs of primers and one exonuclease (exo) probe were designed targeting the conserved region of the cytochrome B (cytb) gene. The designed primer combinations and probe concentration were optimized. The specificity and stability of the method were evaluated by cross-reactivity and repeatability experiments. The sensitivity of the Rt-RAA method and its results for simulated mixed samples and commercial samples were compared with those of the national standard method. The results showed that the Rt-RAA method had strong specificity and good stability and could detect the target sequence in less than 30 min. The detection limit for dog-derived ingredients was 0.2%, which was significantly better than that of real-time fluorescence polymerase chain reaction (PCR). The results of the Rt-RAA method for simulated mixed samples and commercial samples were completely consistent with those of the national standard method. Therefore, Rt-RAA is suitable for the detection of dog-derived ingredients, providing a fast and accurate new method for the identification of meat-derived ingredients.}
}