@article{CUI2024, 
author = {Yanyan CUI and Yongxu YUAN and Mingkun GUO and Wenbing HE and Ming LI and Shichun PEI and Dajun LI},
title = {Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells},
year = {2024},
journal = {Food Science},
volume = {45},
number = {24},
pages = {128-138},
keywords = {Polygonum divaricatum L.polysaccharide, lipopolysaccharide, RAW264.7 cells, inflammation, mitogen-activated protein kinases/nuclear factor kappa B signaling pathway},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20240417-166},
doi = {10.7506/spkx1002-6630-20240417-166},
abstract = {ObjectiveTo explore the in vitro anti-inflammatory activity and action mechanism of a polysaccharide extracted from Polygonum divaricatum L. (PSPDL).MethodsThe structure of PSPDL was characterized using high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and nuclear magnetic resonance spectroscopy (NMR). The in vitro antiinflammatory activity and action mechanism of PSPDL were investigated in lipopolysaccharide (LPS)-induced RAW264.7 cells.ResultsPSPDL had a relative molecular mass of 59.475 kDa and consisted mainly of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara), with a molar ratio of 1.69:4.95:1.04:21.79:19.01:31.68:19.84. PSPDL was an α-pyran polysaccharide containing (1→4)-α-D-Glcp linkage. PSPDL inhibited the release of inflammatory factors and related gene mRNA expression, ameliorated oxidative stress, down-regulated the protein expression levels of p38, p-p38, IκB-α, p65, and p-p65.ConclusionPSPDL exhibited anti-inflammatory activity, perhaps by regulating inflammatory mRNA expression and modulating the mitogen-activated protein kinases/nuclear factor kappa B (MAPK/NF-κB) signaling pathway. This finding provides a scientific basis for the development and utilization of PSPDL resources.}
}