@article{WANG2024, 
author = {Donghua WANG and Xu WANG and Yuting WANG and Shan GAO and Weining WANG and Fuming YANG and Dianyu YU},
title = {Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode},
year = {2024},
journal = {Food Science},
volume = {45},
number = {24},
pages = {259-265},
keywords = {glassy carbon electrode, biosensors, artificial crude soybean oil, phosphatidylcholine, quick detection},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20240508-049},
doi = {10.7506/spkx1002-6630-20240508-049},
abstract = {A biosensor for the determination of phosphatidylcholine (PC) in artificial crude soybean oil was fabricated by immobilizing horseradish peroxidase (HRP) and choline oxidase (ChOx) onto on a glassy carbon electrode (GCE) modified with multi-walled carbon nanotube (MWCNTs), SnO2 and chitosan (CS). Under the conditions of ChOx and HRP addition each at 2.5 U and pH 7.5, the sensor showed significant electrochemical response to PC. PC concentrations in the range of 10–300 mg/L were linearly correlated with the peak current, and the detection limit was 2 mg/L. Recoveries for spiked samples ranged from 90% to 110% with relative standard deviations (RSDs) less than 4.68%. Good correlation was observed between the results of the biosensor and those of high performance liquid chromatography (HPLC) with a correlation coefficient (r) of 0.9929. After 14 days of storage, the sensor still retained 98% of its initial current value, indicating its good stability. This study can provide theoretical support for the rapid inspection and control of vegetable oil refining.}
}