@article{DENG2025, 
author = {Sisi DENG and Hao QIN and Meijin XIONG and Hongyang ZHAO and Ling LIU and Yuying HUANG and Lei BAO and Shaobo LI},
title = {Rapid Determination of 1-Deoxynojirimycin in Milk Powder by Precolumn Derivatization Combined with High Performance Liquid Chromatography with Fluorescence Detection},
year = {2025},
journal = {Food Science},
volume = {46},
number = {4},
pages = {278-284},
keywords = {milk powder, 1-deoxynojirimycin, high performance liquid chromatography, fluorescence detection},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20240812-092},
doi = {10.7506/spkx1002-6630-20240812-092},
abstract = {A rapid precolumn derivatization combined with high performance liquid chromatography (HPLC) with fluorescence detection method for the determination of the 1-deoxynojirimycin (DNJ) content in functional milk powder. Samples were extracted with 60% methanol in water. In pH 8.5 borate buffer solution, o-phthalaldehyde was added as a derivatizing agent to remove the interference from primary amines. DNJ in samples reacted with 9-fluorenylmethoxycarbonyl chloride to form a complex with fluorescence absorption. The separation was performed on a C18 column (150 mm × 4.6 mm, 2.7 μm). Mobile phase A was 10 mmol/L sodium acetate buffer solution, and mobile phase B was acetonitrile. Gradient elution was performed at a flow rate of 1.5 mL/min. The column temperature was 45 ℃. The excitation wavelength was 275 nm. The emission wavelength was 320 nm. Good separation of DNJ from other components was achieved. The detection limit was 4 ng/mL, and the calibration curve was linear in the range of 0.01 to 50 μg/mL, with a determination coefficient (R2) > 0.999. The recovery rates ranged from 101.8% to 104.1%. The method was validated to be stable and reliable, and could be used as an effective method for determining the content of DNJ in milk powder.}
}