@article{LI2025, 
author = {Wei LI and Guodong SUN and Jiarong LU and Zhongkai ZHAO and Jie YANG},
title = {Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction},
year = {2025},
journal = {Food Science},
volume = {46},
number = {6},
pages = {263-274},
keywords = {non-bovine milk, real-time polymerase chain reaction, food adulteration, authenticity analysis},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20240727-273},
doi = {10.7506/spkx1002-6630-20240727-273},
abstract = {In this study, adulteration of bovine milk in fresh and thermally processed non-bovine milk was detected by SYBR Green and TaqMan real-time polymerase chain reaction (real-time PCR), and the effect of different heat treatment methods on the adulteration detection was explored aiming to meet the requirements for the detection of adulteration of different commercial non-bovine milk products. The results showed that the designed bovine-specific primers allowed specific amplification of DNA in bovine milk without cross-reactivity with DNA from non-target animals. The minimum detection limits of SYBR Green and TaqMan real-time PCR for bovine milk DNA were 1 and 10 pg, respectively, and both methods were capable of detecting at least 0.1% (m/m) bovine milk adulteration in non-bovine milk mixtures. The results of nine repeated experiments over three days confirmed high reproducibility of the methods. In addition, using the real-time PCR methods, bovine milk was detected in 7 out of 20 commercial non-bovine milk products, indicating high resolution and great practical application value of these methods.}
}