@article{QIN2025, 
author = {Huimin QIN and Shiqiang YUE and Weijiang YE and Kaizhe WANG and Yuang GAO},
title = {Research Progress on Efficient Biocatalytic Synthesis of D-Allulose},
year = {2025},
journal = {Journal of Food Science and Technology},
volume = {43},
number = {2},
pages = {19-28},
keywords = {D-allulose 3-epimerase, D-allulose, high-throughput screening strategies, immobilization technology, whole-cell catalysis},
url = {https://www.sciopen.com/article/10.12301/spxb202400823},
doi = {10.12301/spxb202400823},
abstract = {D-allulose is a novel low-calorie rare sugar with special physiological functions, including blood sugar regulation and antioxidant effects. In present, the biological synthesis of D-allulose mainly utilized D-allulose 3-epimerase to catalyze the conversion of D-fructose into D-allulose. The large-scale biosynthesis of D-allulose using biological enzymes has primarily focused on improving the catalytic activity, thermal stability, and acid tolerance of D-allulose 3-epimerase to meet industrial production requirements. The research progress on the current status of D-allulose production and the latest advances in its biosynthesis, including molecular-directed modification of isomerases, ultra-high-throughput screening assisted by directed evolution, and novel enzyme immobilization strategies to enhance the industrial properties of D-allulose 3-epimerase were summarized. Furthermore, the application of whole-cell catalytic systems in D-allulose production was also discussed. The aim of this review was to provide a reference for the efficient and green biosynthesis of D-allulose and to propose challenges and possible solutions in industrial production.}
}