@article{Zhang2025, 
author = {Yanjiao Zhang and Chengzhi Mao and Xuyang Liu and Lizhong Guo and Chunhui Hu and Xiaobo Li and Lili Xu and Hao Yu},
title = {Insights into the evolution and mechanisms of response to heat stress by whole genome sequencing and comparative proteomics analysis of the domesticated edible mushroom Lepista sordida},
year = {2025},
journal = {Mycology},
volume = {16},
number = {1},
pages = {324-343},
keywords = {Edible mushroom, Lepista sordida, genome, proteome, heat stress response},
url = {https://www.sciopen.com/article/10.1080/21501203.2024.2363620},
doi = {10.1080/21501203.2024.2363620},
abstract = {Lepista sordida is a valuable edible mushroom rich in natural bioactive compounds. In the present study, a high-quality whole-genome of a domesticated strain of L. sordida was sequenced, revealing a 40.67 Mb genome in 13 contigs. Phylogenetic analysis revealed that L. sordida is evolutionarily closely related to edible mushroom Lyophyllum decastes and Hypsizygus marmoreus. Heat stress has a significant effect on the yield and quality of mushrooms, but the molecular basis for this is poorly understood in L. sordida. A label-free comparative proteomic analysis was performed under different heat stress conditions. The growth of L. sordida mycelia was inhibited, and nuclear apoptosis occurred under heat stress. Ca2+ and MAPK signaling pathways were found to be involved in heat stress signal transduction. It is hypothesized that the expression of various heat shock proteins plays a crucial role in the response to heat stress. In addition, the components of the ubiquitin-proteasome system and the thioredoxin system were upregulated, preventing the accumulation of misfolded proteins and possibly supporting the response to heat stress. In summary, these results provide a fundamental insight into the evolution and heat stress-responsive mechanisms in L. sordida and may facilitate the breeding of heat-tolerant strains for artificial cultivation.}
}