@article{YU2024, 
author = {Miao YU and Xiaoxi XU and Jiaping LÜ and Xiaoyang PANG and Shuwen ZHANG and Yunna WANG},
title = {Membrane Separation Followed by Ion Exchange Chromatography for Preparation of High-Purity α-Lactalbumin},
year = {2024},
journal = {Journal of Dairy Science and Technology},
volume = {47},
number = {4},
pages = {12-18},
keywords = {α-lactalbumin, β-lactoglobulin, separation and purification, membrane separation, ion exchange chromatography},
url = {https://www.sciopen.com/article/10.7506/rykxyjs1671-5187-20240618-044},
doi = {10.7506/rykxyjs1671-5187-20240618-044},
abstract = {In this study, ion exchange chromatography was employed to prepare high-purity α-lactalbumin from whey protein powder obtained from raw cow’s milk by sequential membrane separation and spray drying. The results showed that α-lactalbumin constituted 21.04% of the true protein in the permeate obtained using a 50-nm pore-sized ceramic membrane with three-fold concentration, one cycle of microfiltration and two cycles of washing, which was higher than that obtained using a 100-nm pore-sized ceramic membrane (15.84%). The permeate was spray dried and separated by ion exchange chromatography. Good chromatographic separation of α-lactalbumin and β-lactoglobulin was achieved with increasing NaCl concentration from 0 to 0.5 mol/L at up to 10 column volumes, and 98.18% pure α-lactalbumin and 97.82% pure β-lactoglobulin were obtained. The results of this study provide support for the industrial preparation of high-purity α-lactalbumin.}
}