@article{GAO2024, 
author = {Jiali GAO and Xiaoru WANG and Xiao XIAO and Jia CHEN and Deshang MENG and Wei SUN},
title = {Identification of Peptoniphilus asaccharolyticus Isolated from Spoiled Fermented Milk Intended for Students},
year = {2024},
journal = {Journal of Dairy Science and Technology},
volume = {47},
number = {5},
pages = {7-11},
keywords = {fermented milk intended for students, Peptoniphilus asaccharolyticus, strain identification, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, whole genome sequencing},
url = {https://www.sciopen.com/article/10.7506/rykxyjs1671-5187-20240708-055},
doi = {10.7506/rykxyjs1671-5187-20240708-055},
abstract = {This study focuses on the identification of Peptoniphilus asaccharolyticus from spoiled fermented milk intended for students based on isolation, counting, morphological observation, biochemical identification, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and whole-genome sequencing. The results showed that the isolate grew well on PCA medium under anaerobic conditions and was identified as P. asaccharolyticus on a fully automated microbial identification system with a confidence level of 98%. By MALDI-TOF MS, it was confirmed as P. sasaccharolyticus with a comparison value of 2.241. The result of whole genome sequencing showed an average nucleotide identity (ANI) of 98.58%. The proposed method allows rapid and accurate identification of P. sasaccharolyticus and can meet the requirements of food safety detection.}
}