@article{Pan2025, 
author = {Hao Pan and Yuehong Wang and Xinna Bai and Binjie Liu and Hongzhi Quan and Qingwen Zeng and Jiajia Zeng and Qian Tang and Liqun Gu and Zhiyuan Deng and Zhigang Yao and Ousheng Liu and Zhangui Tang},
title = {MiR-145 affects cell cycles by inhibiting cyclin A2 and cyclin dependent kinase 6 in oral squamous cell carcinoma, a progression and prognostic biomarker},
year = {2025},
journal = {Oral Science and Homeostatic Medicine},
volume = {1},
number = {2},
pages = {9610002},
keywords = {oral squamous cell carcinoma, miR-145, miR-21, prognostic biomarker, CCNA2, CDK6},
url = {https://www.sciopen.com/article/10.26599/OSHM.2025.9610002},
doi = {10.26599/OSHM.2025.9610002},
abstract = {Identifying novel prognostic markers is crucial for predicting clinical outcomes and guiding targeted therapies. This study explores the biomarker potential of specific miRNAs, particularly miR-145 and miR-21, in the prognosis of oral squamous cell carcinoma (OSCC) and their associated mechanisms. mRNA and miRNA expression profiles were analyzed in OSCC patients. Bioinformatic analysis identified miR-145 and miR-21 as key markers. Clinical data from 87 patients showed that high miR-145 and low miR-21 expression correlated with a 91.6% 5-year survival rate. Functional studies showed that miR-145 induced G0/G1 phase arrest and shortened the S phase in OSCC cells. Overexpressing miR-145 or knocking down cyclin A2 (CCNA2) or cyclin-dependent kinase 6 (CDK6) inhibited OSCC cell proliferation. Dual-luciferase assays confirmed that miR-145 directly targets the 3′-UTRs of CCNA2 and CDK6, reducing their expression. MiR-145, particularly when combined with miR-21, is a promising OSCC prognostic biomarker. It exerts its effects by modulating the cell cycle via CCNA2 and CDK6 suppression.}
}