TY  - JOUR
AU  - Yan, Chengyang
AU  - Mei, Xuanhao
AU  - Gong, Xue
AU  - Xu, Weilin
PY  - 2024
TI  - Practical applications of total internal reflection fluorescence microscopy for nanocatalysis
JO  - Industrial Chemistry & Materials
SN  - 2755-2608
SP  - 85
EP  - 99
VL  - 2
IS  - 1
AB  - Fluorescence microscopy has evolved from a purely biological tool to a powerful chemical instrument for imaging and kinetics research into nanocatalysis. And the demand for high signal-to-noise ratio and temporal–spatial resolution detection has encouraged rapid growth in total internal reflection fluorescence microscopy (TIRFM). By producing an evanescent wave on the glass–water interface, excitation can be limited to a thin plane to ensure the measured accuracy of kinetics and image contrast of TIRFM. Thus, this unique physical principle of TIRFM makes it suitable for chemical research. This review outlines applications of TIRFM in the field of chemistry, including imaging and kinetics research. Hence, this review could provide guidance for beginners employing TIRFM to solve current challenges creatively in chemistry.
UR  - https://doi.org/10.1039/d3im00046j
DO  - 10.1039/d3im00046j