TY  - JOUR
AU  - Jang, Seo-Yun
AU  - Yi, Sung Hun
AU  - Song, Hyeon-A
AU  - Park, Min-Ji
AU  - Chung, Kyung-Sook
AU  - Lee, Jong Kil
AU  - Choi, Jae Woong
AU  - Song, Nho-Eul
AU  - Rhee, Young Kyoung
AU  - Lee, Kyung-Tae
PY  - 2026
TI  - Immunostimulatory effects of dietary Lactococcus lactis subsp. lactis KCCM13334P from rice wine on macrophages and cyclophosphamide-induced immunosuppressed mice via toll-like receptor 2
JO  - Food Science and Human Wellness
SN  - 2097-0765
SP  - 9250476
VL  - 15
IS  - 5
AB  - Lactococcus lactis subsp. lactis KCCM13334P (KMGR) was isolated from Makgeolli, a traditional Korean rice wine. The study analyzed the genomic characteristics and performed functional annotation of the complete genome sequence. Results showed that IFN-γ-primed RAW 264.7 cells, treatment with KMGR (6.25, 12.50, or 25.00 μg/mL) significantly enhanced immune response mediator and cytokines production. KMGR significantly activated NF-κB and AP-1 signaling pathways. KMGR improved macrophage activity by stimulating TAK1 and TRAF6 via TLR2 signaling. In CTX-induced immunosuppressed mice, KMGR administration recovered body weight and spleen and mesenteric lymph node indices. KMGR restored CTX-suppressed natural killer cell activity and populations of immune cells. Additionally, KMGR repaired the CTX-induced histological structure of the colon and the expression of tight junction proteins, mucin-2, and TLR2. KMGR treatment maintained a gut microbiota composition similar to the control groups. Taken together, our data suggest that KMGR shows potential as a functional postbiotic with immune-enhancing activity.
UR  - https://doi.org/10.26599/FSHW.2025.9250476
DO  - 10.26599/FSHW.2025.9250476