@article{CHEN2023, 
author = {Chen CHEN and Rui ZHANG and Yuxin WANG and Tonghe ZHANG and Zilun ZHANG and Wenshen JIA and Wei ZHOU},
title = {Establishment of a Fluorescence-Based Quantitative Real-Time PCR Method for Detection for Caprine-Derived Ingredients in Milk Powder},
year = {2023},
journal = {Journal of Dairy Science and Technology},
volume = {46},
number = {6},
pages = {20-24},
keywords = {adulteration detection, goat milk powder, horse milk powder, real-time fluorescence quantitative polymerase chain reaction},
url = {https://www.sciopen.com/article/10.7506/rykxyjs1671-5187-20231224-062},
doi = {10.7506/rykxyjs1671-5187-20231224-062},
abstract = {This study established a fluorescence-based quantitative real-time polymerase chain reaction method for the detection of caprine-derived ingredients in milk powder. The specificity, sensitivity, and stability of the caprine-specific primers were evaluated. By linear fitting of the difference in cycle threshold (ΔCt) as a function of the mixing proportion between goat and horse milk powder, a calibration curve for the relative quantitation of caprine-derived ingredients in milk powder was established as follows: y = 0.7209x + 5.6519 (R2 = 0.9875), and the minimum detection limit of this method was 0.0001 ng/μL. The recoveries of the proposed method were 96.22%-112.00%, and the inter- and intra-group coefficient of variation was ≤ 0.79% and ≤ 1.77%, respectively.}
}