@article{Wang2024, 
author = {Xia Wang and Han Zhan and Bin Mou and Libo Hua and Zheng Zhao and Lang Huang and Jian Sima and Zongqin Xiang and Yong U. Liu},
title = {Chronic in vivo imaging of tau-induced neurodegeneration and microglial response using two-photon microscopy},
year = {2024},
journal = {Aging Research},
volume = {2},
number = {3},
pages = {9340035},
keywords = {neurodegeneration, microglia, two-photon in vivo imaging, tau},
url = {https://www.sciopen.com/article/10.26599/AGR.2024.9340035},
doi = {10.26599/AGR.2024.9340035},
abstract = {Investigations into neurodegenerative progression have traditionally relied on pathological sectioning and intergroup analysis, with outcomes heavily dependent on sample size. Emerging in vivo imaging techniques now enable dynamic, long-term tracking of the same subjects, enhancing comparability and reducing variability. In this study, we use two-photon microscopy to visualize neurons and microglia through fluorescent markers introduced by adeno-associated virus (AAV) labeling and transgenic mice. Additionally, AAV-driven tau pathology induces neurodegeneration in the brain. A head ring is attached to the mouse’s skull for reversible mounting on a head-fixed treadmill. This setup allows us to image neurodegeneration progression without anesthesia, quantifying changes in the number, morphology, and spatial positioning of tau-overexpressing neurons and microglia. This approach provides spatiotemporal insights into neurodegeneration previously unattainable.}
}