@article{Yu2025, 
author = {Shenping Yu and Yangchao Shi and Yanbing Lv and Li Wang and Yuke Ma and Ning Li and Xiuyuan Zuo and Yifan Li and Jinjin Fan and Zifeng Zhang and Ruili Wu and Huaibin Shen and Lin Song Li},
title = {Construction of a magnetic driven-quantum dots ratiometric fluorescence-immunosensor for rapid and accurate detection of C-reactive protein},
year = {2025},
journal = {Nano Research},
volume = {18},
number = {9},
pages = {94907207},
keywords = {ratiometric fluorescence, magnetic-fluorescent quantum dots, magnetic driven immunosensor, rapid and accurate},
url = {https://www.sciopen.com/article/10.26599/NR.2025.94907207},
doi = {10.26599/NR.2025.94907207},
abstract = {Efficient, sensitive, and convenient quantum dots (QDs) fluorescent immunosensors have a wide range of applications in the field of in vitro diagnostics (IVD). However, traditional single-signal readout methods are susceptible to interference, have limited sensitivity, and involve time-consuming detection processes. These have become key bottlenecks restricting their use in point-of-care (POC) detection. Herein, we developed a novel immunosensor based on QDs ratiometric fluorescence (QRF) and magnetic driven technology for sensitive, rapid, accurate detection of C-reactive protein (CRP). High-quality magnetic-fluorescent QDs nanobeads (QBs) were prepared by efficiently and orderly layered loading of magnetic nanoparticles (MNP) and red QDs using mesoporous silica nanoparticles (MSN) as a template. The magnetic driven-QRF-immunosensor was constructed based on green QDs probes-functionalized microplate. The results showed that the linear range of magnetic-fluorescence probes for the detection of CRP was 0.1–1000 ng/mL, with a sensitivity of up to 0.05 ng/mL. Compared to conventional QRF-immunosensor, the detection time was halved (within 30 min), and the sensitivity improved by approximately 5-fold. Recovery experiments and clinical sample analyses also demonstrated the good accuracy of this immunoassay. Therefore, the constructed novel magnetic driven-QRF-immunosensor shows great potential for clinical diagnosis and disease monitoring, and is expected to be applied in POC detection as a new generation of detection technology.}
}