TY  - JOUR
AU  - Ren, Jing
AU  - Liu, Mengli
AU  - Rong, Mingjie
AU  - Zhang, Xuan
AU  - Wang, Gang
AU  - Liu, Yihan
AU  - Li, Haijun
AU  - Duan, Shichao
PY  - 2025
TI  - The pros and cons of mechanical dissociation and enzymatic digestion in patient-derived organoid cultures for solid tumor
JO  - Cell Organoid
SN  - 3007-6552
SP  - 9410009
VL  - 1
IS  - 1
AB  - Patient-derived organoids (PDOs) are revolutionizing cancer research, serving as invaluable models for tumor biology and therapeutic screening. The fidelity and applicability of these organoids are fundamentally shaped by the tissue dissociation techniques employed, namely mechanical dissociation and enzymatic digestion. This comprehensive review delves into the nuances of these two methods, scrutinizing their effects on solid tumor organoid properties, including stemness, heterogeneity, long-term culturing. We discuss the advantages and limitations of each technique, with a focus on their impact on tumor microenvironment preservation, their application in drug screening and cancer modeling. Moreover, we examine how recent technological breakthroughs have bolstered the efficiency and scalability of organoid production through these methods. Our analysis is designed to assist researchers in choosing the optimal tissue dissociation strategy for their research objectives and to fuel the evolution of organoid-based cancer models.
UR  - https://doi.org/10.26599/CO.2024.9410009
DO  - 10.26599/CO.2024.9410009