@article{YUAN2023, 
author = {Lie YUAN and Weiying ZHOU and Duanfang ZHOU and Xu LIU and Xiaoli LI},
title = {Celastrol exerts anti-ER+ breast cancer by activating endoplasmic reticulum stress to induce apoptosis},
year = {2023},
journal = {Journal of Army Medical University},
volume = {45},
number = {19},
pages = {2065-2073},
keywords = {celastrol, ER+ breast cancer, endoplasmic reticulum stress, apoptosis},
url = {https://www.sciopen.com/article/10.16016/j.2097-0927.202304042},
doi = {10.16016/j.2097-0927.202304042},
abstract = {ObjectiveTo investigate the mechanism of celastrol（Cel）against estrogen receptor positive（ER+）breast cancer by activating endoplasmic reticulum stress-mediated apoptosis.MethodsER+ human breast cancer cell lines MCF-7 and T47D were used as research objects. MTT assay was used to detect the effect of Cel on cell viability. Western blotting were employed to detect the expression of apoptosis-related proteins. RT-qPCR and Western blotting were applied to measure the expression of endoplasmic reticulum stress-related molecules at mRNA and protein levels. A nude mouse model of orthotopic transplantation of MCF-7 cells was established to observe the effect of Cel on the growth of transplanted tumors.ResultsMTT results showed that Cel inhibited the viability of MCF-7 and T47D cells in a dose-dependent manner（P<0.05）. Western blotting showed that Cel up-regulated the expression of apoptosis-related proteins, Cleaved PARP and BAX, and down-regulated the expression of BCL-2（P<0.05）. RT-qPCR indicated that Cel up-regulated the mRNA levels of endoplasmic reticulum stress-related genes IRE1α, ATF6, PERK, ATF4, CHOP and BIP（P<0.05）. Western blotting revealed that Cel treatment resulted in increased expression levels of endoplasmic reticulum stress marker BIP, of PERK pathway related proteins p-PERK, p-eIF2α, ATF4 and CHOP, of IRE1α pathway associated proteins p-IRE1α and XBP1s, and of ATF6 pathway associated protein Cleared ATF6. In vivo experiment results showed that Cel significantly reduced the volume of orthotopic transplanted tumor in nude mice（P<0.05）, and had no significant effect on their body weight.ConclusionCel has a certain inhibitory effect on the proliferation of ER+ breast cancer MCF-7 and T47D cells, and its mechanism may be related to the activation of endoplasmic reticulum stress signaling pathway to induce apoptosis.}
}