@article{CHEN2023, 
author = {Huan CHEN and Jinhe XU and Yuekai WANG and Danni YIN and Bing WU and Hongli LI},
title = {Mechanism of photosensitive STIM1 calcium channel driven retinal injury stress depression},
year = {2023},
journal = {Journal of Army Medical University},
volume = {45},
number = {11},
pages = {1131-1141},
keywords = {retinal damage, stress depression, photosensitive modification STIM 1, behavioristics, demyelination},
url = {https://www.sciopen.com/article/10.16016/j.2097-0927.202301055},
doi = {10.16016/j.2097-0927.202301055},
abstract = {ObjectiveTo investigate the occurrence of stress depression caused by retinal cells death driven by activation of photosensitive STIM1 calcium channel and the changes in brain inflammation related to the visually-associated brain regions.MethodsA mouse model of retinal injury driven by light was constructed by injecting STIM1 virus into the vitreous body of mouse eyeball. HE staining and depression-related behavior test were performed to assess the severity of retinopathy and incidence of depression. Reverse transcription-PCR（RT-PCR）, Western blotting and immunofluorescence assay were used to analyze the changes of microglial cells, inflammatory factors（IL-6 and MIP-1α）, nerve cell damage marker（S100B）, apoptotic protein（Caspase-3）and myelin basic protein（MBP）in the depressive mice.Results①The cell density in each layer of the mouse retina were decreased significantly under the light mode of 3 h/d for 7 consecutive days（P<0.05）. ②In the open field test, the times of entering the central area, moving distance in the central area and total distance of movement were obviously decreased（P<0.05）, the index of sucrose preference of the sucrose preference test was significantly decreased（P<0.01）, and the immobile time in the forced swimming test and the tail suspension test was notably increased（P<0.001）in the mice from the photosensitive group. The positive rate of depression in all the 4 tests（open field test, sucrose preference test, forced swimming test and tail suspension test）was 23.26%. ③The density of IBa-1+ cells in the visual cortex and dorsolateral geniculate nucleus was elevated in the depressive mice of the photosensitive group（P<0.05）. The expression of IL-6 and MIP-1α at mRNA level was obviously elevated（P<0.000 1）, as well as the expression of S100B and Caspase-3 at protein level was significantly enhanced（P<0.001）, while the MBP protein in the white matter was clearly weakened（P<0.05）. The intensity of MBP fluorescence signal was evidently reduced in the corpus callosum（cc）area and cingulate gyrus（cg）area in white matter（P<0.000 1）.ConclusionA stress depression-like mouse model caused by light-driven retinal injury is successfully constructed. Pathogenesis of depression is closely associated with inflammatory environment and neuronal apoptosis in the vision-related brain regions and demyelinating injury in white matter.}
}